Backcrossing
（重定向自Backcross）
![This image demonstrates backcrossing of a heterozygous mouse from one genetic background onto another genetic background. In this example, the gene knockout is performed on 129/Sv cells and then backcrossed into the C57B/6J genetic background. With each successive backcross, there is an increase in the percentage of C57B/6J DNA that constitutes the genome of the offspring.](/Images/godic/202412/22/Backcrossing_mice_from_chimera.svg0955.png")
Backcrossing is a crossing of a hybrid with one of its parents or an individual genetically similar to its parent, in order to achieve offspring with a genetic identity which is closer to that of the parent. It is used in horticulture, animal breeding and in production of gene knockout organisms.