美国手语（American Sign Language，简称ASL）是美国、加拿大英语地区及墨西哥部分地区里，最常用的手语。一如其他手语，美国手语的文法和语法都和当地的口语不尽相同。目前还没有可靠的调查，证实美国手语的母语使用人口；一般估计在二十万至二百万人之间。

美国手语在菲律宾、新加坡、香港、科特迪瓦、布基纳法索、加纳、多哥、贝宁、尼日尔利亚、乍得、加蓬、刚果民主共和国、中非共和国、毛里塔尼亚、肯雅、马达加斯加及津巴布韦等地也有用户。

American Sign Language (ASL) is the predominant sign language of Deaf communities in the United States and most of anglophone Canada. Besides North America, dialects of ASL and ASL-based creoles are used in many countries around the world, including much of West Africa and parts of Southeast Asia. ASL is also widely learned as a second language, serving as a lingua franca. ASL is most closely related to French Sign Language (LSF). It has been proposed that ASL is a creole language, although ASL shows features atypical of creole languages, such as agglutinative morphology.