Myrosinase
（重定向自Thioglucosidase）
![](/Images/godic/202502/16/1e4m5033.png")
![Figure 2: Mechanism of glucosinolate hydrolysis by myrosinase.[2]](/Images/godic/202502/16/Myrosinase_general_mechanism5033.png")
![Figure 3: Active site of myrosinase during the first step of glucosinolate hydrolysis. Here, ascorbate is used as a cofactor to substitute for the missing second catalytic glutamate in order to cleave the thio-linked glucose.[3]](/Images/godic/202502/16/Glucosinolate_hydrolysis_with_ascorbate_cofactor_at_active_site_of_myrosinase5033.png")
Myrosinase (EC3.2.1.147, thioglucoside glucohydrolase, sinigrinase, and sinigrase) is a family of enzymes involved in plant defense against herbivores. The three-dimensional structure has been elucidated and is available in the PDB (see links in the infobox).