吡哆醛激酶（英语：pyridoxal kinase；EC2.7.1.35）在酶学中是一个催化如下化学反应的酶：

因此，此酶的两个底物分别是ATP与吡哆醛，而它的两个产物则是ADP与吡哆醛5'-磷酸。

此酶属于转移酶家族，特异性地转移含磷基团（磷酸转移酶）并并以醇基作为受体。此酶类的系统命名为ATP：吡哆醛5'-磷酸转移酶。通常使用的其他名称包括吡哆醛激酶（磷酸化）、吡哆醛5-磷酸激酶、吡哆醛磷酸激酶以及吡哆醇激酶。此酶参与维生素B₆代谢。

In enzymology, a pyridoxal kinase (EC2.7.1.35) is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are ATP and pyridoxal, whereas its two products are ADP and pyridoxal 5'-phosphate.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:pyridoxal 5'-phosphotransferase. Other names in common use include pyridoxal kinase (phosphorylating), pyridoxal 5-phosphate-kinase, pyridoxal phosphokinase, and pyridoxine kinase. This enzyme participates in vitamin B₆ metabolism.