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词汇 Oligonucleotide array sequence analysis
分类 英语词汇 英语翻译词典
释义

Oligonucleotide array sequence analysis

中文百科

DNA微数组 DNA microarray

(重定向自Oligonucleotide array sequence analysis)
举例来说,这是一个有40,000个杂交探针点阵寡核苷酸的DNA微数组,并有放大图显示细节。
微阵列原理
Hybridization of the target to the probe.
The steps required in a microarray experiment.

DNA微数组(DNA microarray)又称DNA数组DNA芯片,比较常用的名字是基因芯片(gene chip)。是一块带有DNA微阵列(microarray)的特殊玻璃片或硅晶元片,在数平方公分之面积上布放数千或数万个核酸探针;检体中的DNA、cDNA、RNA等与探针结合后,借由荧光或电流等方式侦测。经由一次测验,即可提供大量基因串行相关信息。它是基因组学和遗传学研究的工具。研究人员应用基因芯片就可以在同一时间定量的分析大量(成千上万个)的基因表现,具有快速、精确、低成本之生物分析检验能力。

英语百科

DNA microarray DNA微阵列

(重定向自Oligonucleotide array sequence analysis)
Hybridization of the target to the probe.
The steps required in a microarray experiment.
Two Affymetrix chips. A match is shown at bottom left for size comparison.
A DNA microarray being printed by a robot at the University of Delaware

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles (10 moles) of a specific DNA sequence, known as probes (or reporters or oligos). These can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA (also called anti-sense RNA) sample (called target) under high-stringency conditions. Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target.

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