Methylglyoxal synthase
![Crystal structure (PDB ID: 1EGH) of methylglyoxal synthase homohexamer with residues of one active site highlighted. Three active sites exists within the three major grooves. Image generated with PyMOL.](/Images/godic/202501/27/Methylglyoxal_synthase_with_active_site_highlighted1305.png")
![Active site residues of methylglyoxal synthase (Lys23, Thr45, Thr48, Gly66, His19, His98, Asp71). Image generated from crystal structure (PDB ID: 1EGH) with PyMOL.](/Images/godic/202501/27/Methylglyoxal_synthase_active_site1305.png")
![Methylglyoxal synthase arrow-pushing mechanism. Recreated on ChemDraw and adapted from Saadat, D; Harrison, D. H. (1999).](/Images/godic/202501/27/Methylglyoxal_synthase_mechanism1305.png")
In enzymology, a methylglyoxal synthase (EC4.2.3.3) is a enzyme that catalyzes the chemical reaction
Hence, this enzyme has one substrate, DHAP, and two products, methylglyoxal and phosphate. Attempts to observe reversibility of this reaction have been unsuccessful.