dNaM

dNaM is an artificial nucleotide. It pairs up with d5SICS in a hydrophobic interaction. It was not able to be removed by the error-correcting machinery of the E. coli it was inserted into. The pairing of d5SICS–dNaM is mediated by packing and hydrophobic forces instead of hydrogen bonding, which occurs in natural base pairs. Therefore, in free DNA, rings of d5SICS and dNaM are placed in parallel planes instead of the same plane.